Neuro-Protective Effect and the Mechanism of Semen Euryales Active Fraction Against H2 O2-Induced PC12 Cells
10.14148/j.issn.1672-0482.2016.0157
- VernacularTitle:芡实正丁醇部位分离组分对 H2 O2诱导的 PC12细胞的神经保护作用及其机制研究
- Author:
Xiu-He FAN
1
;
Qi-Nan WU
;
Shi-Lin DAI
;
Yue-Song GONG
;
Wei YUE
;
Xu-Ran LI
;
Hui-Qin XU
;
Jin-Gao YU
Author Information
1. 南京中医药大学药学院江苏省中药资源产业化过程协同创新中心
- Keywords:
active fraction of semen euryales;
oxidative stress;
PC12 cells;
neuroprotection
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(2):157-160
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:OBJECTIVE To investigate the effect of n-butanol extract from Euryale ferox Salisb.against H2 O2-induced ox-idative injury of PC12 cells and explore the protective mechanism of this active components against Alzheimer's disease cell model.METHODS The n-butanol extracts of semen euryales were separated by the column chromatography of polyamide. PC12 cells were cultivated in vitro and treated with 200μmol/L H2 O2 for 8 h to establish the oxidative damage model.Cell via-bilities were determined by MTT assay,and the activities of superoxide dismutase(SOD) and the content of malondialdehyde (MDA) were analyzed in cell-culture medium by the corresponding kits.RT-qPCR was used to detect the mRNA expression of APP,BACE-1 and GSK-3β.RESULTS Compared with the model group,the 50%,70%,95% concentration of alcohol in n-butanol components of semen euryales improved the cell viability of PC12(P <0.05).The 70% alcohol components signifi-cantly increased SOD activity and decreased the content of MDA in culture supernatants(P <0.05)and significantly down-reg-ulated the expression of GSK-3β(P <0.01).CONCLUSION The 70% and 95% alcohol components of n-butanol extracts of semen euryales shows significant protective effects on oxidative stress-induced cell injury.The protection mechanism may be related to the increased SOD activity,the reduced the emission of MDA,and the inhibition the key genes associated with the accumulation of Aβ.
