Identification of Age-associated Proteins and Functional Alterations in Human Retinal Pigment Epithelium
- Author:
Jin XIUXIU
1
;
Liu JINGYANG
;
Wang WEIPING
;
Li JIANGFENG
;
Liu GUANGMING
;
Qiu RUIQI
;
Yang MINGZHU
;
Liu MENG
;
Yang LIN
;
Du XIAOFENG
;
Lei BO
Author Information
1. Henan Eye Institute,Henan Eye Hospital,People's Hospital of Zhengzhou University,Henan Provincial People's Hospital,Zhengzhou 450003,China;Branch of National Clinical Research Center for Ocular Disease,Henan Provincial People's Hospital,Zhengzhou 450003,China;School of Medicine,Henan Provincial People's Hospital,Henan University,Zhengzhou 450003,China
- Keywords:
Human retinal pigment epithelium;
Proteomics;
Aging;
Retina;
Apoptosis
- From:
Genomics, Proteomics & Bioinformatics
2022;20(4):633-647
- CountryChina
- Language:Chinese
-
Abstract:
Retinal pigment epithelium(RPE)has essential functions,such as nourishing and sup-porting the neural retina,and is of vital importance in the pathogenesis of age-related retinal degen-eration.However,the exact molecular changes of RPE during aging remain poorly understood.Here,we isolated human primary RPE(hRPE)cells from 18 eye donors distributed over a wide age range(10-67 years old).A quantitative proteomic analysis was performed to analyze changes in their intracellular and secreted proteins.Age-group related subtypes and age-associated proteins were revealed and potential age-associated mechanisms were validated in ARPE-19 and hRPE cells.The results of proteomic data analysis and verifications suggest that RNF123-and RNF149-related protein ubiquitination plays an important role in protecting hRPE cells from oxidative damage dur-ing aging.In older hRPE cells,apoptotic signaling-related pathways were up-regulated,and endo-plasmic reticulum organization was down-regulated both in the intracellular and secreted proteomes.Our work paints a detailed molecular picture of hRPE cells during the aging process and provides new insights into the molecular characteristics of RPE during aging and under other related clinical retinal conditions.
