microRNA-30d can inhibit the proliferation, migration and invasion of human mesothelial cell MSTO-211H
10.3760/cma.j.cn.121094-20200831-00507
- VernacularTitle:microRNA-30d对人恶性胸膜间皮瘤细胞MSTO-211H增殖和迁移以及侵袭的抑制作用
- Author:
Xiuyuan YUAN
1
;
Fangfang ZHANG
;
Yali HUANG
;
Zhenyu JIA
;
Li JU
;
Yun XIAO
;
Hailing XIA
;
Yanan GAO
;
Min YU
;
Man YU
;
Xing ZHANG
;
Lijin ZHU
Author Information
1. 310013 杭州医学院（浙江省医学科学院）公共卫生学院
- Keywords:
Cell proliferation;
Apoptosis;
Cell migration;
Invasion;
Malignant pleural mesothelioma;
miR-30d
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(2):99-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect and molecular mechanism of microRNA-30d (miR-30d) in the process of proliferation, migration and invasion of malignant mesothelioma cell line MSTO-211H.Methods:In April 2017, the human MSTO-211H cells was used to establish miR-30d overexpressed MSTO-211H cell model by transfection of miR-30d mimics. The qRT-PCR was performed to detect the expression level of miR-30d in the cells transfected miR-30d mimics. The effects of miR-30d on the proliferation, apoptosis, migration and invasion of MSTO-211H cells were analyzed by CCK-8 experiment, flow cytometry, cell scratch experiment and Transwell method.Results:After transfection of miR-30d, the expression level of miR-30d in the MSTO-211H+miR-30d cells group was significantly higher than MSTO-211H+miR NC cells group ( P<0.01) . The cell activity of MSTO-211H+miR-30d group (105.13%±2.35%) was significantly lower than MSTO-211H+miR NC cells group (115.40%±1.35%) , and the level of apoptosis (3.97%±0.36%) was significantly higher than MSTO-211H+miR NC cells group (1.47%±0.10%) ( P<0.01) . The relative migration areas at 12 and 24 h of MSTO-211H+miR-30d cells group (9.35±3.16 μm 2 and 58.19±1.82 μm 2) were significantly lower than MSTO-211H+miR NC cells group (54.42 ±5.26 μm 2 and 88.32 ±1.96 μm 2) ( P<0.01) . Compared with the MSTO-211H+miR NC cells group, the numbers of cell migration and cell invasion were reduced in the MSTO-211H+miR-30d cells group ( P<0.01) . Conclusion:miR-30d can regulate the progression of malignant pleural mesothelioma by inhibiting the proliferation, apoptosis, migration and invasion of MSTO-211H cells.
