Determination of trace bismuth by atomic fluorescence spectrometry in blood

VernacularTitle:血中痕量铋测定的原子荧光光谱法
Author: Lezhou ZHOU ¹ ; Sheng FU ; Shouquan GAO ; Guowen HE
Author Information

1. 湖南省职业病防治院
Keywords: Spectrometry,fluorescence; Blood; Bismuth
From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2016;34(12):939-941
CountryChina
Language:Chinese
Abstract: Objective To establish a method for determination trace bismuth in blood by atomic fluorescence spectrometry (AFS).Methods 4.0 ml nitric acid and 1.0 ml perchloric acid was added into 1.0 ml blood sample then through automation graphite digestion instrument digested,after that 1.0 ml thiocarbamidevitamin (10％) was injected,8％ HC1 constant volume to 10.0 ml,the bismuth was detected by atomic fluorescence spectrometry with 5.0 ml digestive sample.Results The method showed a linear relationship within the range of 0.4-50.0 μg/L (r=0.999 7).The within-run and between-run relative standard deviations (RSD) of repetitive measurement at 10.0,20.0,40.0 μg/L concentration levels were 2.2％-4.9％ and 3.0％-4.0％.The detection limit was 0.032 μg/L.The recoveries of bismuth were 93.0％-103.9％.Conclusion This method is low detection limit,good accurate and high sensitivity.It has been applied for determination of trace bismuth in blood samples those who need occupation health examination or poisoning diagnosis.