Fabrication and evaluation of hydroxyapatite-chitosan scaffold via simulated body fluid biomimetic mineralization
10.7518/hxkq.2016.01.002
- VernacularTitle:模拟体液仿生矿化法制备的羟磷灰石-壳聚糖支架的性能研究
- Author:
Ke XU
1
;
Yanhong ZHAO
;
Hongfa LI
Author Information
1. 天津医科大学口腔医院正畸科
- Keywords:
biomimetic mineralization;
hydroxyapa-tite;
chitosan
- From:
West China Journal of Stomatology
2016;(1):6-11
- CountryChina
- Language:Chinese
-
Abstract:
?????Objective???This?research?aimed?to?fabricate?a?hydroxyapatite?(HA)-chitosan?scaffold?via?simulated?body?fluid?(SBF)?biomimetic?mineralization?and?determine?how?mineralization?time?affects?scaffold?construction?and?cell?compatibility.?Methods???The?HA-chitosan?scaffolds?were?fabricated?by?freeze-drying?technique?and?then?subjected?to?precalcification,?also?known?as?alternative?soaking?method.?Afterward,?precalcificated?scaffolds?were?placed?into?the?SBF?to?conduct?the?minerali-zation?process.?Mineralization?time?was?set?at?7,?14,?and?21?days,?corresponding?to?three?experimental?groups.?Pure?chitosan?scaffolds?acted?as?the?control?group,?and?the?physical?and?chemical?properties?of?the?four?groups?were?tested.?Osteogenic-induced?adipose-derived?stem?cells?(ADSCs)?were?seeded?into?the?scaffolds?to?investigate?the?scaffolds’?cell?compatibility.?Results???The?mineral?substance?of?the?14-day?group?exhibited?a?uniform?distribution.?The?crystal?composition?of?the?mineral?substance?suited?the?HA’s?features.?The?compressive?elastic?modulus?increased?along?with?the?extension?of?mineralization?time.?The?21-day?group?showed?a?statistically?significant?increase?in?compressive?elastic?modulus?compared?with?the?control?group?(P<0.05).?The?cell?proliferation?level?of?the?14-day?group?was?significantly?the?highest?among?the?three?experimental?groups?(P<0.05).?The?calcium?ion?and?the?typeⅠcollagen?had?the?highest?secretion?amount?when?the?cells?were?seeded?into?the?14-day?group.?Conclusion???The?SBF?biomimetic?mineralization?method?can?be?used?to?fabricate?HA-chitosan?bone-tissue-engineering?scaffolds.?The?biological?compatibility,?as?well?as?the?chemical?and?physical?properties,?reached?the?optimum?levels?at?day?14.
