Effect of topically applied recombinant adenovirus of adiponectin on bone defect repair
- VernacularTitle:局部应用脂联素基因重组腺病毒对骨缺损修复的影响
- Author:
Xue LÜ
1
;
Na LIU
;
Wen DU
;
Jiayang LI
;
Yue SUN
;
En LUO
Author Information
- Keywords: adiponectin; adenovirus; bone defect; osteogenesis
- From: West China Journal of Stomatology 2015;(4):361-365
- CountryChina
- Language:Chinese
- Abstract: Objective??To?investigate?the?effect?of?local?administration?of?recombinant?adenovirus?of?human?adiponectin?(hAPN)?Ad-hAPN-EGFP?on?tibial?defect?repair?of?SD?rats. Methods??Tibial?defect?(2?mm)?models?of?36?SD?rats?(72?sides)?were?randomly?divided?into?three?groups?(A,?B,?and?C;?n=24).?The?three?groups?were?injected?with?Ad-hAPN-EGFP,?Ad-EGFP,?and?normal?saline,?respectively,?during?operation?and?the?day?after?operation.?A?week?after?the?operation,?the?expres-sion?of?hAPN?and?osteogenesis-related?factors?were?detected?by?real-time?polymerase?chain?reaction.?Three?weeks?after?operation,?the?tibias?were?examined?by?micro-computed?tomography,?hematoxylin-eosin?staining,?and?Masson?staining?to?evaluate?the?restoration?of?bone?defects.?Results??1)?The?hAPN?expression?was?detected?in?group?A?but?not?in?groups?B?and?C.?Osteogenesis-related?factors?expression?of?group?A?was?significantly?higher?than?that?of?the?other?groups?(P<0.05).?2)?Osteogenesis?(including?bone?mineral?density,?relative?bone?volume,?trabecular?number?and?trabecular?thickness)?in?group?A?were?more?evident?than?those?in?groups?B?and?C?(P<0.05).?No?significant?differences?were?found?between?groups?B?and?C?(P>0.05).?Conclusion??Local?administration?of?recombinant?adenovirus?Ad-hAPN-EGFP?may?be?an?effective?strategy?to?improve?the?restoration?of?bone?defects?in vivo.
