Quercetin protects against lipopolysaccharide-induced cardiac injury in mice
10.3969/j.issn.1673-4254.2015.07.27
- VernacularTitle:槲皮素对内毒素性心肌损伤的保护作用及机制
- Author:
Jian LI
1
;
Jian ZHANG
;
Xinmin DONG
;
Huafei DENG
;
Fan YANG
Author Information
1. 湘南学院基础医学部
- Keywords:
quercetin;
endotoxin;
cardiac injury
- From:
Journal of Southern Medical University
2015;(7):1068-1072
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect of quercetin against lipopolysaccharide (LPS)-induced cardiac injury in mice. Methods C57BL/6J mice were randomized into 4 groups to receive intraperitoneal injection of saline (negative control) or LPS (20 mg/kg), or fed with quercetin (100 mg/kg for 7 days) with or without subsequent LPS injection (quercetin+LPS group and quercetin control group, respectively). Six hour after LPS injection, the mice were tested for cardiac function with an echocardiograph, and the protein expressions of Bax, Bcl-2, iNOS, and eNOS in the myocardium were evaluated with Western blotting;serum NO concentration was also measured. The survival of the mice within 5 days after LPS injection was recorded to draw the survival curve. Results Quercetin pretreatment significantly improved the cardiac function of LPS-challenged mice (P<0.05), and attenuated LPS-induced increment in myocardial iNOS expression and decrement in eNOS level. LPS significantly increased the myocardial Bax expression and slightly decreased Bcl-2 expression; quercetin pretreatment decreased Bax expression to the control level and significantly lowered Bax/Bcl-2 ratio as compared with the LPS group. Serum NO level was significantly increased by nearly 2.5 folds in LPS-challenged mice, but was markedly decreased with quercetin pretreatment (P<0.05). The 5-day survival rate of LPS-treated mice was 10%, which was increased to 45% in quercetin-pretreated mice (P<0.05). Conclusion Quercetin can alleviate LPS-induced cardiac dysfunctions in mice to increase their survival rate following LPS challenge.
