Effect of indomethacin on BCR/ABL-Wnt/β-catenin pathway in K562 cells
10.3969/j.issn.1673-4254.2015.07.12
- VernacularTitle:吲哚美辛对K562细胞株BCR/ABL-Wnt/β-catenin信号通路的影响
- Author:
Zhangling LIU
1
;
Jing HU
;
Zhenglan HUANG
;
Hui LI
;
Xin LIU
;
Wenli FENG
Author Information
1. 重庆医科大学检验医学院临床检验诊断学教育部重点实验室
- Keywords:
indomethacin;
chronic myelogenous leukemia;
K562 cells;
BCR/ABL;
Wnt/β-catenin;
pathway
- From:
Journal of Southern Medical University
2015;(7):998-1002
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of indomethacin on the proliferation and Wnt/β-catenin pathway in K562 cells. Methods The cell growth of K562 cells treated with different concentrations of indomethacin was assessed with MTT assay, and the colony-forming ability of the cells was evaluated by colony-forming assay. The mRNA expressions of BCR/ABL andβ-catenin were detected by RT-PCR, and the protein expressions of pBCR/ABL, total BCR/ABL,β-catenin, pGSK-3βand c-myc were analyzed by Western blotting. Results Indomethacin significantly suppressed the growth and colony-forming ability of K562 cells in a dose-dependent manner. Indomethacin treatment dose-dependently decreased the protein level of pBCR/ABL and total BCR/ABL without affecting bcr-abl mRNA expressions. Compared with the control groups, indomethacin-treated cells showed obviously decreased mRNA and protein expressions ofβ-catenin and decreased protein expressions of pGSK-3βand c-myc. Conclusion Indomethacin inhibits the proliferation of K562 cells by suppressing the activity of bcr-abl-Wnt/β-catenin pathway.
