Effects of acute hypervolemic hemodilution and intraoperative blood salvage on erythrocyte morphology and biomechanics

VernacularTitle:高容量血液稀释复合回收对红细胞形态与力学性能的影响
Author: Yiwen ZHANG ¹ ; XINHZumin ; Qiang GUO ; Zhongcheng HE
Author Information

1. 南方医科大学附属顺德第一人民医院麻醉科
Keywords: atomic force microscopy; erythrocyte; blood transfustion; autologous
From: Journal of Southern Medical University 2015;(7):966-970
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of acute hypervolemic hemodilution (AHH) and intraoperative blood salvage (IOBS) on the morphology and biomechanics of erythrocytes using atomic force microscopy (AFM). Methods Blood samples were collected from 20 patients undergoing orthopedic surgery before operation (T1), immediately after AHH (T2), and after blood salvage (T3). AFM at nanometer resolution was used to examine the morphology and biomechanics of the collected erythrocytes. Results The blood samples collected at T1 showed a significantly lower ratio of heteromorphous erythrocytes than those collected at T2 and T3 [(2.6 ± 1.3)%vs (19.3 ± 1.6)%and (17.6 ± 1.4)%, P<0.05]. AFM revealed significant differences in the morphology and biomechanics of the single erythrocyte in salvaged blood and blood after AHH compared with those of venous blood. Conclusion AHH and IOBS may cause significant changes in the morphology and biomechanics of erythrocytes in the salvaged blood.