Effects of propofol on pulmonary metastasis of intravenously injected MADB106 tumor cells and expression of E-cadherin andβ-catenin in rats
- VernacularTitle:丙泊酚对肿瘤细胞肺转移及E钙粘蛋白、β-连环蛋白表达的影响
- Author:
Wei WANG
1
;
Chunshui LIN
;
Yajing ZHANG
;
Ying CHEN
;
Peipei GUO
Author Information
1. 南方医科大学附属南方医院麻醉科
- Keywords:
propofol;
tumor metastatic;
E-cadherin;
β-catenin
- From:
Journal of Southern Medical University
2015;(6):852-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different doses of propofol on pulmonary metastasis of intravenous injected MADB106 tumor cells and the expression of E-cadherin and β-catenin in the metastatic tumor tissue in rats. Methods Forty Fischer 344 male rats were randomly divided into 4 groups (n=10) for intravenous administration of normal saline, intralipid, or propofol at 30 or 50 mg/kg via the femoral vein. One hour after the infusion, MADB106 tumor cells (2 × 105) were injected intravenously in the rats. Three weeks later, pulmonary metastasis tumor foci and metastatic inhibitory rate were observed and the expression of E-cadherin and β-catenin in the metastatic tumor tissue were detected by immunohistochemistry. Results Compared with the normal saline group, intralipid group showed no significant differences in the number of metastatic tumor foci in the lungs or E-cadherin and β-catenin expressions (P>0.05), which were all significantly lowered in the two propofol groups (P<0.05 or 0.01). The dose of propofol was inversely correlated with the number of metastasis tumor foci (r=-0.879) and expressions of E-cadherin (r=-0.755) and β-catenin (r=-0.693) (P<0.01). Conclusion Propofol can dose-dependently suppress pulmonary metastasis of intravenous injected MADB106 tumor cells by inhibiting the Wnt/β-catenin pathway and down-regulating E-cadherin andβ-catenin expressions in the metastatic tumor tissue.
