Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs.
10.4142/jvs.2011.12.3.273
- Author:
Md Rafiqul ALAM
1
;
Joong Ryong JI
;
Min Su KIM
;
Nam Soo KIM
Author Information
1. Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. alammr74@yahoo.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
MMP-2;
osteoarthritis;
synovial fluid;
TIMP-2;
TRAP
- MeSH:
Acid Phosphatase/analysis/blood;
Animals;
Arthritis, Experimental/enzymology/etiology/veterinary;
Biological Markers/*analysis/*blood;
Blotting, Western/veterinary;
Dislocations/complications/*veterinary;
Dog Diseases/*enzymology/etiology;
Dogs;
Enzyme-Linked Immunosorbent Assay/veterinary;
Female;
Isoenzymes/analysis/blood;
Male;
Matrix Metalloproteinase 2/analysis/blood;
Osteoarthritis/enzymology/etiology/*veterinary;
Spectrophotometry/veterinary;
Stifle/physiopathology;
Synovial Fluid/*enzymology;
Tissue Inhibitor of Metalloproteinase-2/analysis/blood
- From:Journal of Veterinary Science
2011;12(3):273-280
- CountryRepublic of Korea
- Language:English
-
Abstract:
The levels of tartrate resistant acid phosphatase (TRAP), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in synovial fluid (SF) and serum in cases of canine osteoarthritis (OA) were measured. OA was induced by a surgically-created medial patellar luxation in the left stifle of 24 dogs. SF and blood samples were collected at 1.5- and 3-month intervals, respectively. Every 3 months, one dog was euthanatized to collect tissue samples from both stifles. TRAP levels in SF and serum were measured using a spectrophotometer, and TRAP-positive cells in joint tissues were identified by enzyme histochemistry. MMP-2 and TIMP-2 in SF and serum were detected by Western blotting and ELISA, respectively. TRAP in SF from the stifles and serum was significantly increased (p < 0.05) after 3 months. TIMP-2 in SF and serum was significantly decreased (p < 0.05), whereas MMP-2 in SF was significantly increased (p < 0.05) during the progression of OA. Histochemistry revealed an increased number of TRAP-positive cells in tissues from OA-affected joints. Assays measuring TRAP, MMP-2, and TIMP-2 in SF and serum, and methods that detect increased numbers of TRAP-positive cells in the joint tissues can play an important role in identifying the early phases of degenerative changes in canine joint components.
