Regulatory T cells in the treatment of autoimmune myositis in mice：efficacy and mechanism
- VernacularTitle:调节性T细胞治疗实验性小鼠多发性肌炎及机制研究
- Author:
Qiang SHI
1
;
Chenglin TIAN
;
Jiexiao LIU
;
Chuanqiang PU
Author Information
1. 中国人民解放军总医院海南分院神经内科
- Keywords:
autoimmune myositis;
CD4+CD25+Foxp3+Tregs;
flow cytometry;
IL-10;
TGF-β;
PD-1;
CTLA-4
- From:
Journal of Southern Medical University
2015;(4):602-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of CD4+CD25+Foxp3+Tregs in the treatment of autoimmune myositis (EAM) in mice and explore the possible mechanisms. Methods Mouse models of EAM were divided randomly into model group and treatment group, and the latter received infusion of CD4 + CD25 + Foxp3 + Tregs separated from normal mouse spleen by magnetic activated cell sorting. The changes of muscle pathology was observed, and the expression of PD-1 and CTLA-4 in spleen CD4+ CD25+ Foxp3+ Tregs was analyzed using flow cytometry; peripheral blood IL-10 and TGF-β levels were tested using double antibody sandwich ELISA. Results Compare with the model group, the mice in the treatment group showed significantly reduced muscular inflammatory cell infiltration, increased blood levels of IL-10 and TGF-β (P<0.05), and increased expression of PD-1 and CTLA-4 in spleen CD4+CD25+Foxp3+Tregs (P<0.05). Conclusion CD4+CD25+Foxp3+Tregs reinfusion produces therapeutic effect in mice with EAM by increasing peripheral blood IL-10 and TGF-βlevels and PD-1 and CTLA-4 expressions in spleen CD4+CD25+Foxp3+Tregs.
