Role of RAGE in lipopolysaccharide-induced cytoskeletal changes in mouse pulmonary mi-crovascular endothelial cells
10.3969/j.issn.1673-4254.2015.01.02
- VernacularTitle:晚期糖基化终产物受体在脂多糖介导小鼠肺微血管内皮细胞骨架变化中的作用
- Author:
Xiaoyan ZHOU
1
;
Weijin ZHANG
;
Qiaobing HUANG
;
Xiaohua GUO
Author Information
1. 南方医科大学病理生理学教研室//广东省医学休克微循环重点实验室
- Keywords:
mouse pulmonary microvascular endothelial cells;
lipopolysaccharide;
receptor,advanced glycation end products;
cytoskeleton
- From:
Journal of Southern Medical University
2015;(1):6-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate lipopolysaccharide (LPS)-induced changes of cytoskeletal filamentous actin in primary isolated pulmonary microvascular endothelial cells (PMVECs) from wild-type and RAGE knock-out mouse. Methods The lungs of wild-type and RAGE knock-out mice were digested with collagenase type I to obtain endothelial cells purified by anti-CD31-coupled magnetic beads. The PMVEC identified by factor VIII labeling were stimulated with LPS at different concentrations and the changes of filamentous actin were observed by confocal microscopy. Results The cultured primary cells showed typical endothelial cell phenotype as examined with factor VIII labeling. LPS stimulation caused rearrangement of the cytoskeletal filament F-actin in wild-type mouse PMVECs with stress fiber formation, but such changes were not obvious in RAGE knock-out mouse PMVECs. Conclusion Mouse PMVECs of a high purity can be obtained by immune magnetic beads. RAGE is involved in LPS-induced destruction of mouse PMVEC cytoskeletons.
