Effects of Biejiajian Pills on Wnt signal pathway signal moleculesβ-catenin/TCF4 complex activities and downstream proteins cyclin D1 and MMP-2 in hepatocellular carcinoma cells
10.3969/j.issn.1673-4254.2014.12.10
- VernacularTitle:鳖甲煎丸对Wnt信号通路中β-catenin/TCF4复合物活性及信号分子cyclin D1、MMP-2的影响
- Author:
Bin WEN
1
;
Haitao SUN
;
Songqi HE
;
Yang CHENG
;
Wenyan JIA
;
Eryan FAN
;
Jie PANG
Author Information
1. 中国人民解放军第四五八医院中医科
- Keywords:
hepatocellular carcinoma;
Biejiajian Pills;
β-catenin/TCF4 complex;
cyclin D1;
MMP-2
- From:
Journal of Southern Medical University
2014;(12):1758-1762
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma. Methods HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR. Results Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities ofβ-catenin/TCF4 complex. Conclusion Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.
