Effects of selenium supplement on atherogenesis of ApoE-knockout mice fed high fat diet
10.3760/cma.j.issn.0253-3758.2016.03.011
- VernacularTitle:补硒对载脂蛋白E基因敲除小鼠动脉粥样硬化的影响
- Author:
Hualei DONG
1
;
Na YUAN
;
Tao SUN
;
Aishe DUN
;
Haifeng HOU
Author Information
1. 山东省泰山医院心内科
- Keywords:
Atherosclerosis;
Selenium;
Lipid peroxidation
- From:
Chinese Journal of Cardiology
2016;44(3):244-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of selenium supplement on atherogenesis and endothelial function in ApoE-knockout mice fed high fat diet.Methods ApoE-knockout mice fed with selenium-deficient and high fat diet were randomly allocated into 3 groups based on random number table including control group (not supplied with sodium selenite,n =10),low dosage selenium supplement group (supplied water with 0.1 mg/L sodium selenite,n =10) and high dosage selenium supplement group (supplied water with 0.2 mg/L sodium selenite,n =10).After 15 weeks,the following values were measured:the concentrations of selenium in heart and liver,the levels of serum lipid,the parameters of antioxidant function including activities of superoxide dismutase(SOD) and glutathion peroxidase (GSH-Px) and malondialdehyde (MDA) level in serum,the parameters of endothelial function including serum nitric oxide (NO),endothelin 1(ET-1),and vascular endothelial growth factor (VEGF) levels,and ET-1 and VEGF levels in aorta roots.The atherosclerotic lesions in aorta roots were analyzed with oil red O staining.Results (1) The selenium concentrations in heart and liver were significantly higher in high dosage and low dosage selenium supplement groups compared to control group (both P ＜ 0.05).(2) The levels of triglyceride,total cholesterol,low density lipoprotein cholesterol,very low density lipoprotein cholesterol and high-density lipoprotein cholesterol were similar among groups (all P ＞ 0.05).(3) The activity levels of serum SOD were significantly higher in low dosage ((113.8 ± 12.5) U/ml) and high dosage selenium supplement group ((152.3 ± 11.3) U/ml) compared to control group ((90.7 ± 10.7) U/ml,all P ＜ 0.05).The activity levels of serum GSH-Px were significantly higher in low dosage ((53.9 ± 7.2) U/ml) and high dosage ((69.6 ± 8.7) U/ml) selenium supplement groups than that of control group ((36.4 ± 5.6) U/ml,all P ＜0.05).The serum MDA levels in low dosage ((4.73 ± 1.05) nmol/ml) and high dosage ((4.13 ± 1.21)nmol/ml) selenium supplement groups were significantly lower than that of control group ((5.97 ± 1.08) nmol/ml,all P ＜ 0.05).(4) The serum NO concentrations in low dosage ((61.5 ± 12.8) μmol/L) and high dosage ((79.0 ± 14.6) μmol/L) selenium supplement groups were significantly higher than that of control group((42.7 ± 9.1) μmol/L,all P ＜ 0.05).The concentrations of serum ET-1 in low dosage ((52.8 ±6.3)ng/L)and high dosage ((46.3 ±4.7)ng/L)selenium supplement groups were significantly lower than that of control group((72.2±6.3)ng/L,P ＜ O.05).The concentrations of serum VEGF in low dosage ((97.4 ± 16.5) ng/L) and high dosage ((83.5 ± 22.0) ng/L) selenium supplement groups were significantly lower than that of control group((125.8 ± 18.6) ng/L,P ＜0.05).The expression levels of ET-1 and VEGF in aorta roots among low dosage and high dosage selenium supplement groups were significantly lower compared to control group (all P ＜ 0.05).(5) The plaque area of aorta roots in low dosage ((0.95 ± 0.19)× 105 μm2) and high dosage selenium supplement ((0.75 ± 0.15) × 105 μm2) groups were significantly smaller than that of control group ((1.13 ± 0.23) × 105 μm2),and the plaque area in high dosage selenium supplement group was significantly smaller than in low dosage selenium supplement group (P ＜ 0.05).Conclusion Supplement of selenium can attenuate atherogenesis in ApoE-knockout mice fed high fat diet,which is possibly mediated via reducing lipid peroxidation and improving endothelial functions.
