Transfusion of necrotic cells redresses regulatory T cell and Th17 cell imbalance in septic mice
10.3969/j.issn.1673-4254.2014.07.08
- VernacularTitle:坏死细胞逆转脓毒症小鼠Treg/Th17比例的失衡
- Author:
Dongsheng GU
1
;
Lu XIONG
;
Yanping HAN
;
Erwei SUN
Author Information
1. 南方医科大学第三附属医院风湿免疫科
- Keywords:
sepsis;
regulatory T cells;
Th17 cells;
necrotic cells
- From:
Journal of Southern Medical University
2014;(7):956-960
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transfusion of necrotic cells on regulatory T (Treg) and Th17 cell balance in septic mice. Method Thirty-four C57BL/6 mice were randomized into PBS group (n=5), sham-operated group (n=5), sepsis group (n=12), and necrotic cell transfusion group (n=12) and subjected to intraperitoneal PBS injection, sham operation by separating the cecum only, cecal ligation and puncture (CLP), and injection of 2 × 107 necrotic cells 5 days before CLP, respectively. All the mice were sacrificed 2 weeks after CLP for analyzing the proportion of CD4+Foxp3+Treg cells and CD4+IL17A+Th17 cells in the peripheral blood, spleen and thymus by flow cytometry. Results The percentage of Th17 cells and Treg/Th17 ratio in the spleen was significantly higher in CLP group than in the sham-operated group and PBS group (P<0.01). The percentage of Treg cells in the thymus was significantly lower in CLP group than in the sham-operated group (P<0.01). Pre-infusion of necrotic cells redressed the abnormality of Treg and Th17 cell percentages and Treg/Th17 imbalance in mice following CLP (P<0.05). Conclusion Pre-infusion of necrotic cells can reverse Treg/Th17 imbalance in septic mice.
