Impact of All-trans Retinoic Acid on Gene Expression Profile of Glioblastoma Cell Line SHG-44
10.3321/j.issn:1000-467X.2008.05.007
- VernacularTitle:全反式维甲酸对胶质瘤SHG-44细胞基因表达谱的影响
- Author:
Yi ZENG
1
;
Zhong YANG
;
Yang-Yun HAN
;
Chao YOU
Author Information
1. 四川大学华西医院
- Keywords:
All-trans retinoic acid;
Astrocytoma;
SHG-44 cells;
Differentially expressed genes;
cDNA microarray
- From:Chinese Journal of Cancer
2008;27(5):482-490
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND & OBJECTIVE: Astrocytoma has the trend of malignant progression. Differentiation-inducing therapy can induce tumor differentiation and make tumor cells become less malignant or even normal. This study was to investigate the impact of all-trans retinoic acid (ATRA) on the gene expression profile of glioblastoma cell line SHG-44, and to provide basic data for further research on gene therapy for human astrocytoma. METHODS: After treatment of 10靘ol/L ATRA, total RNA was extracted from SHG-44 cells for reverse transcription-polymerase chain reaction, and cDNA product was marked with fluorochromes Cy3 and Cy5. The gene expression profiles of SHG-44 cells before and after treatment of ATRA were detected by chip hybridization to identify differentially expressed genes. Some differentially expressed genes were selected randomly for Northern blot analysis. RESULTS: Forty-two differentially expressed genes were found by cDNA microarray: 28 were up-regulated and 14 were down-regulated in ATRA-treated SHG-44 cells as compared with those in untreated SHG-44 cells. These genes were functionally classified into several groups as follow: apoptosis, cell mobility and metastasis, cell cycle and growth regulation, cytoskeleton, differentiation, metabolic pathway, oncogene, oxidative phosphorylation, receptors and signal transduction, ribosome, ubiquitin-proteasome system, growth factor and cytokine, and so on. CONCLUSIONS: ATRA can result in the changes of gene expression profiles in SHG-44 cells. These differentially expressed genes may mediate the mechanism of ATRA-induced differentiation of SHG-44 cells, and regulate tumor progression.
