An enrichment method of EBV in blood samples by F beads

VernacularTitle:F磁珠对血液样本中EB病毒的富集方法
Author: Hao ZHENG ¹ ; Jinxing LU ; Xiaoping CHEN
Author Information

1. 中国疾病预防控制中心传染病预防控制所　传染病预防控制国家重点实验室，北京　102206
Keywords: F beads; EBV detection; M beads
From: Chinese Journal of Experimental and Clinical Virology 2023;37(5):537-542
CountryChina
Language:Chinese
Abstract: Objective:To achieve the effect of EBV enrichment from different anticoagulants, a novel recombinant protein F was designed and produced.Methods:A novel recombinant protein F was designed, which contained human IL-2 signal peptide, his, human IgG1 Fc, human Ficolin-2 neck region and Fibrinogen-like domain (FGD). The synthetic gene segment was cloned into pCDNA?3.1(+ ) vector, which was then transfected into HEK293F cells. The recombinant protein (F protein) was then produced in supernatant. F beads was constructed by coating F protein onto protein A beads. At last, EBV positive whole blood samples anticoagulated by three kinds of anticoagulants were collected. F beads and M beads (previously studied) were compared to be applied to enrich EBV from these blood samples.Results:M beads were effective to enrich EBV from heparin or sodium citrate anticoagulated blood ( P<0.01), not for EDTA anticoagulated blood. While, F beads were effective for all three anticoagulated blood ( P<0.01). Conclusions:F beads could enrich EBV from different anticoagulated blood.