Comparison of features of occult hepatitis B virus infection between blood donors and patients with chronic hepatitis B
10.3760/cma.j.cn112866-20221130-00247
- VernacularTitle:献血者和慢性乙肝患者隐匿性乙肝病毒感染特征比较
- Author:
Kewang SUN
1
;
Hao WANG
;
Chao LIU
;
Tianqi JIANG
;
Xiangyan HUANG
Author Information
1. 潍坊医学院医学检验学院，潍坊 261041
- Keywords:
Blood donor;
Occult hepatitis B virus infection;
serum markers;
S gene;
mutation
- From:
Chinese Journal of Experimental and Clinical Virology
2023;37(4):373-376
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the serological and molecular biological characteristics and differences of occult hepatitis B infection (OBI) between blood donors and chronic hepatitis B (CHB) patients.Methods:Nineteen OBI samples from blood donors and Nineteen OBI samples from CHB patients were collected, and named as group A and group B, respectively. Chemiluminescence method was used for hepatitis B five items detection. Real-time PCR method was used for HBV DNA quantification, and S gene was amplified by nested PCR and sequenced by DNA sequencing.Results:The level of HBV DNA in group A was significantly lower than that in group B ( P<0.05), and there were no significant differences in the serological result between the two groups. The mutation rate in the " a" determinant of S gene in group B was significantly higher than that in group A ( P<0.05). Conclusions:Compared with CHB patients, OBI blood donors have lower levels of viral replication and less chance of S gene mutation, and the use of highly sensitive nucleic acid detection reagents and method during blood screening can maximize the safety of blood transfusion.
