miRNA-144 in the saliva is a genetic marker for early diagnosis of esophageal cancer
10.3969/j.issn.1673-4254.2013.12.16
- VernacularTitle:唾液中miRNA-144可作为早期诊断食道癌的基因标志物
- Author:
Weidong WU
1
;
Wenjin HOU
;
Zhefan WU
;
Yuejun WANG
;
Yongsheng YI
;
Weijia LIN
Author Information
1. 暨南大学第四附属医院//广州市红十字会医院心胸外科
- Keywords:
saliva;
miRNA-144;
esophageal cancer;
early diagnosis
- From:
Journal of Southern Medical University
2013;(12):1783-1786
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the presence of miRNA-144 in the saliva of patients with esophageal cancer and its value for early diagnosis of esophageal cancer. Methods Saliva samples were collected form patients with esophageal cancer admitted in the Fourth Affiliated Hospital of Jinan University and the First Affiliated Hospital of Guangzhou Medical College between January, 2011 and May, 2013, with saliva samples from 50 middle-aged healthy volunteers matched for age and gender ratio as the control group. The contents of miRNA-144 in the samples were detected with RT-PCR. Results The levels of miRNA-144 in both the whole saliva and saliva supernatant were significantly higher in esophageal cancer group than in the control group (P<0.05). In the whole saliva, the cut-off point of miRNA-144 was≥100, with a sensitivity of 74.6%and a specificity of 92.0%for esophageal cancer diagnosis (Az=0.865); in saliva supernatant, the cut-off point was ≥20 with a sensitivity of 53.7% and a specificity of 94.0%(Az=0.754), suggesting a moderate diagnostic value of miRNA-144 in whole saliva and saliva supernatant. Conclusion miRNA-144 is highly expressed in the saliva of patients with esophageal cancer and can be used as a genetic marker for early diagnosis of esophageal cancer.
