Expression of NLRP3 inflammasome in BALB/c mice with imiquimod-induced psoriasis-like inflammation and therapeutic effect of mustard seed (Sinapis Alba Linn)
10.3969/j.issn.1673-4254.2013.09.30
- VernacularTitle:NLRP3炎症小体在咪喹莫特诱导小鼠银屑病样模型中的表达及芥菜籽对其的影响
- Author:
Jian HU
1
;
Runping YANG
;
Chunmiao WEN
;
Hengjin LI
;
Hua ZHAO
Author Information
1. 解放军总医院皮肤科
- Keywords:
mustard seed;
NLRP3 inflammasome;
imiquimod;
psoriasis-like inflammation
- From:
Journal of Southern Medical University
2013;(9):1394-1398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of NLRP3 inflammasome in imiquimod-induced psoriasis-like inflammation in mice and the therapeutic effects of mustard seed (Sinapis Alba Linn). Methods Thirty BALB/c mice were randomized equally into blank control group (fed with normal forage and treated with vehicle), model group (fed with normal forage and treated with 5% imiquimod cream), and experimental group (fed with 5% mustard seed forage and treated with 5% imiquimod cream). RT-PCR was used to detect the mRNA expression of NLRP3, ASC, caspase-1, and caspase-11. Immunohistochemistry was performed to determine the expression and distribution of ASC and caspase-1. ELISA was used to test the serum levels of interleukin-1β (IL-1β) and IL-18. Results Compared with the blank control group, the mice with imiquimod-induced psoriasis-like inflammation showed significantly increased NLRP3, ASC, caspase-1, and caspase-11 mRNA expressions, ASC and caspase-1 protein expressions , and serum levels of IL-1βand IL-18 (P<0.05). These changes were obviously attenuated by feeding the mice with mustard seed. Conclusion NLRP3 inflammasome is involved in imiquimod-induced psoriasis-like inflammation in mice, and mustard seed may suppress the inflammation induced by IL-1βand IL-18 through down-regulating the expression of NLRP3 inflammasome.
