The molecularly etiological study on the human parainfluenza viruses caused an acute respiratory infection outbreak in Shenyang
10.3760/cma.j.cn112866-20210906-00162
- VernacularTitle:沈阳地区一起人副流感病毒3型引起的急性呼吸道感染暴发病原分子流行病学研究
- Author:
Bing WANG
1
;
Min FU
;
Ye CHEN
;
Lina BAI
;
Ying QI
;
Tianbao LI
;
Xinmeng WANG
;
Jiayuan LIANG
Author Information
1. 沈阳市疾病预防控制中心，沈阳 110031
- Keywords:
Human parainfluenza viruses;
Acute respiratory infection;
Outbreak
- From:
Chinese Journal of Experimental and Clinical Virology
2022;36(2):155-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the etiology and genetics of the human parainfluenza virus type 3 (HPIV3) virus which caused an acute respiratory tract infection outbreak in a primary school in Shenyang.Methods:Throat swab samples were collected from 17 students of the primary school where the epidemic of acute respiratory infection outbreak in December 2020 in Shenyang, Liaoning province. TaqMan low-density arrays (TLDA) real-time PCR was performed to simultaneously detect multiple respiratory pathogens. The HN gene was amplified using nested RT-PCR and sequenced, followed by phylogenetic analysis for those HPIV3 positive samples.Results:Of the 17 specimens, 10 were HPIV3 positive by TLDA Real-time PCR, and were accompanied by conditional pathogen infection, consequently, amplification result ed in 7 complete HN sequences. Phylogenetic analysis showed that the infected HPIV3 virus of the outbreak belonged to HPIV subtype C3a. All the 7 strains detected in this study belonged to subbranch C3a.1 evolutionary branch, with a nucleotide homology of 99.9%, a nucleotide homology of 94.56 with the prototype strain Wash/47885/57 and 99.5% with the most phylogenetically close strain of ZJ/11-s-165/KP690785/CHN/11.Conclusions:The HPIV3 virus caused the acute respiratory tract infection outbreak in Shenyang in 2020 and HPIV subtype C3a1 was detected firstly in Northeast China.
