Expression of PD-L1 in cervical squamous cell carcinoma and its immunomodulatory effect
10.3760/cma.j.cn112866-20210209-00031
- VernacularTitle:PD-L1在宫颈鳞状细胞癌中的表达及免疫调控作用
- Author:
Dehui CHEN
1
;
Yingjie YANG
;
Yafei WANG
;
Qinghua SUN
;
Jie REN
Author Information
1. 贵州医科大学临床医学院，贵阳 550004
- Keywords:
Cervical squamous cell carcinoma;
PD-L1;
Human papillomavirus 16;
Immunoregulation
- From:
Chinese Journal of Experimental and Clinical Virology
2021;35(4):384-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To disclose the expression and correlation of E6, E7 and PD-L1 in cervical squamous cell carcinoma (CSCC) tissues and explore the immune regulation of E6, E7 on PD-L1.Methods:The expressions of E6, E7 and PD-L1 in human papillomavirus (HPV) negative normal cervical tissue, cervical intraepithelial neoplasia (CIN) tissue and HPV16, 52 and 58 positive CSCC tissue were detected by Western blot (WB). HPV16 positive CSCC and HPV negative CSCC tissues were used for primary cell isolation, identification and culture. E6, E7 small interfering RNAs (siRNAs) were constructed and transfected into HPV16 positive CSCC cells via liposomes.The expression of PD-L1 in the cells was detected by WB. Plasmids of E6, E7 gene were constructed and overexpressed in HPV negative CSCC cells to detect the expression of PD-L1.Results:The expression of E6, E7 and PD-L1 was not detected in the HPV negative normal cervical. While in the HPV16, 52, 58 positive CSCC tissue group, the expression of all the three were significantly higher than that in the CIN group ( P<0.01). Primary HPV16 positive CSCC and HPV negative CSCC cells were successfully isolated and cultured, which were named as H16CC cell lines and HNCC cell lines respectively. After inhibiting the expression of E6, E7 in H16CC cell lines, the expression of PD-L1 was significantly decreased ( P<0.05). The expression of PD-L1 was significantly increased after the overexpression of E6, E7 in HNCC cell lines ( P<0.05). Conclusions:The expression of PD-L1 is positively correlated with E6, E7, which may regulate the occurrence and development of CSCC by mediating the immune escape mechanism of PD-L1 in CSCC.
