Differential transactivation of HIV-1 subtypes B and C by Tat lysine 41
10.3760/cma.j.cn112866-20190725-00118
- VernacularTitle:HIV-1 B、C亚型Tat第41位赖氨酸对HIV-1转录活化的影响
- Author:
Yu LIU
1
;
Yue HU
;
Xiaohong KONG
Author Information
1. 天津医科大学朱宪彝纪念医院检验科 天津市内分泌研究所 国家卫生健康委员会激素与发育重点实验室 天津市代谢性疾病重点实验室 300134
- Keywords:
Human immunodeficiency viruses;
Trans-activation of transcription;
Transcription;
Subtype B;
Subtype C
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):406-410
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of trans-activation of transcription (Tat) lysine 41 on HIV-1 transcription and compare the effect of HIV-1 subtypes B and C.Methods:The effects of wildtype Tat, Tat K41A and Tat K41R mutants on HIV-1 transcription were assessed by luciferase assay, and the differences of Tat K41 effect were compared in subtypes B and C viruses. Next, immunoprecipitation (IP) assay was used to detect whether Tat K41 of HIV-1 clades B and C affects the interaction between Tat and SIRT1. Finally, Western blot was used to detect whether K41 of Tat B and Tat C affects the acetylation level of p65 K310 in the nucleus.Results:Mutants of subtype C Tat K41 significantly inhibited Tat transactivation, reduced the interaction between SIRT1 and Tat C and decreased the level of p65 K310ac in the nucleus compared with wildtype of subtype C Tat, but there were no significant changes in subtype B Tat.Conclusions:Subtype C Tat K41 plays a vital role in HIV-1 transcription.
