Evaluation on sensitivity of nine rapid influenza viral antigen diagnostic tests
10.3760/cma.j.cn112866-20200330-00099
- VernacularTitle:流感病毒抗原快速检测试剂灵敏度评价
- Author:
Haiwei ZHOU
1
;
Donglai LIU
;
Tingting MA
;
Sihong XU
Author Information
1. 中国食品药品检定研究院传染病诊断试剂二室，北京 100050
- Keywords:
Influenza;
RIDTs;
Analytical sensitivity
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(4):440-443
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the analytical sensitivity of nine rapid influenza virus antigen diagnostic tests (RIDTs).Methods:Human seasonal influenza viruses were collected and amplified. Gene sequencing was performed and TCID 50 were tested. Evaluation of nine RIDTs for the analytical sensitivity was performed after the subsequent dilution of human seasonal influenza A H3N2, H1N1, and influenza B viruses. Results:RIDTs offers lower sensitivity than the nucleic acid amplification test. RIDTs overall had different analytical sensitivity based on the detection of the same isolate, and the difference between result was more than 10 1 TCID 50/ml. Each RIDT had variable levels of positivity with different influenza subtypes/lineages. Conclusions:The study demonstrated that the analytical sensitivity of RIDTs varies.
