Preparation of monoclonal antibodies against severe acute respiratory syndrome coronavirus 2
10.3760/cma.j.cn112866-20200417-00135
- VernacularTitle:严重急性呼吸综合征冠状病毒2单克隆抗体的制备
- Author:
Yingying WANG
1
;
Chao WU
;
Xia XIAO
;
Conghui WANG
;
Ying WANG
;
Geng WANG
;
Yan XIAO
;
Li GUO
;
Lili REN
;
Jianwei WANG
Author Information
1. 北京协和医学院 中国医学科学院病原生物学研究所 100730；北京协和医学院 中国医学科学院病原生物学研究所 国家卫生健康委病原系统生物学重点实验室 100730；中国医学科学院呼吸道疾病病原组研究重点实验室，北京 100730
- Keywords:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2);
Nucleocapsid protein;
Monoclonal antibody;
detection;
Immunofluorescence
- From:
Chinese Journal of Experimental and Clinical Virology
2020;34(3):236-240
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare monoclonal antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) for developing antigen diagnostics.Methods:The supernatants of hybridoma cell lines secreting monoclonal antibodies against SARS-CoV were collected and were subjected to examinate the reaction between the monoclonal antibodies and SARS-CoV-2 N protein by using western blot and indirect enzyme-linked immunosorbent assay (ELISA). The reactive monoclonal antibodies were used to detect SARS-CoV-2 in Vero cell cultures by using immunofluorescence assay.Results:Two monoclonal antibodies, termed A1 and A2, were obtained from the hybridoma cell lines. They can both react with SARS-CoV-2 N protein, while A2 can also recognize SARS-CoV N protein. These monoclonal antibodies can be used in ELISA and immunofluorescence experiments.Conclusions:Two monoclonal antibodies against SARS-CoV-2 N protein were obtained, providing materials for future development of SARS-CoV-2 antigen detection.
