Study of human papillomavirus type 52 gene mutation and lineage distribution collected in Beijing,China
10.3760/cma.j.issn.1003-9279.2016.06.015
- VernacularTitle:北京地区收集的人乳头瘤病毒52型基因突变和型下分类的研究
- Author:
Ying LI
1
;
Ling ZHOU
;
Qinping LIAO
;
Hongtu LIU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所卫生部医学病毒和病毒病重点实验室
- Keywords:
Cervical cancer;
Human papillomavirus type 52;
Mutation;
Lineage
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(6):558-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the variants presence and intratype lineages distribution of Human papillomavirus type 52 (HPV52) in Chinese women.Methods A total of 1 244 cervical specimens were collected.After DNA extraction and genotyping,79 HPV52 single positive DNA specimens were analyzed for E7 oncogenes and Long Control Region (LCR) fragments sequence variations.Finally,50 samples were sequenced for both of the two fragments.Constituent ratio of HPV52 Lineage distribution was compared between mainland China and other regions of Asia.Results DNA variation of E7 and LCR were detected in 92.0％ (46/50) and 96.0％ (48/50) of the cases respectively.Five and fourteen variants of HPV52 E7 and LCR were found respectively.Lineage B was the most prevalent lineage across all of the HPV52 single infection cases (90.0％,45/50),lineage A accounted for 8.0％ (4/50),and lineage C accounted for 2.0％ (1/50),and no lineage D was found.Constituent ratio of HPV52 Lineage distribution was different in different regions of Asia.Conclusions There is a high frequency of mutation in HPV52 E7 and LCR in China.Constituent ratio of HPV52 Lineage distribution of Asia has the character of geographical dependence.
