Immunogenicity of dendritic cells pulsed with Ad5-HIVgag/env in mice
10.3760/cma.j.issn.1003-9279.2016.04.008
- VernacularTitle:负载Ad5-HIVgag/env的DC疫苗在小鼠体内的免疫原性研究
- Author:
Chao LIU
1
;
Xiaozhou HE
;
Xue LIU
;
Ke XU
;
Yi ZENG
;
Xia FENG
Author Information
1. 100124,北京工业大学生命科学与生物医学工程学院病毒与药理室
- Keywords:
Dendritic cell vaccine;
Cell therapy;
Adenovirus;
HIV-1
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(4):369-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate HIV Gag/Env specific cellular immune responses induced in mice immunized with mouse dendritic cells pulsed with Ad5-HIVgag/env.Methods BALB/c mouse DCs were generated by culture of adherent bone marrow cells in the presence of several cytokines.The purity of matured DCs was determined by flow cytometry.DC vaccines were prepared by loading them with replication-deficient recombinant adenovirus Ad5-HIVgag or Ad5-HIVenv.BALB/c mice were immunized by intramuscular injection of above DC vaccines alone or in combination,at different time points post immunization HIV specific cellular immune responses detected by ELISPOT assay.Results Mouse DC vaccines loaded with Ad5-HIVgag/env were generated successfully.Expression of HIV Gag or Env antigens in DC vaccines could be detected by immunofluorescence assay and flow cytometry;and high levels of HIV-specific cellular immune responses were induced in mice immunized with above DC vaccines Conclusions Dendritic cells loaded with Ad5-HIVgag/env could elicit potent HIV specific cellular immune responses in mice.
