Establishment and application of fluorescent antibody virus neutralization test for Hantavirus
10.3760/cma.j.issn.1003-9279.2016.03.017
- VernacularTitle:汉坦病毒荧光抗体中和试验方法的建立
- Author:
Qi LI
1
;
Yamei WEI
;
Xu HAN
;
Zhanying HAN
;
Yanbo ZHANG
;
Yonggang XU
;
Shunxiang QI
Author Information
1. 河北省疾病预防控制中心
- Keywords:
Hemorrhagic fever with renal syndromes;
Hantavirus;
Fluorescent antibody virus neutralization
- From:
Chinese Journal of Experimental and Clinical Virology
2016;30(3):324-327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a fluorescent antibody virus neutralization (FAVN) test for hantavirus (HV).Methods With the method of dilution serum and fixed virus,the serum samples were diluted with different concentration,and with virus-liquid were mixed in 96 culture plate hole,37 ℃ for 1 hour.Then the mixture of serum and virus was added to Veto-E6 cells which had grown into a monolayer,and cultivated in the 37 ℃,5％ CO2 incubator for 7 days; the 96-well plate was fixed and stained.The results were observed under a fluorescence microscope.The neutralization titer was calculated according to the formula.Results The suitable cell concentrations is 4 × 104 ～ 4.5 × 104/hole,and the proper temperature for neutralize is 37 ℃.30 serum specimens of HFRS were tested by FAVN and PRNT,the FAVN is more sensitive and stable.Conclusion FAVN is easier,faster and more sensitive than the others.
