Detection of HPV and MMTV in breast cancer cases

VernacularTitle:乳腺癌患者中HPV和MMTV的基因检测
Author: Chen YAN ¹ ; Yunxin CHEN ; Zhiping TENG ; Danhua SHEN ; Jintao LI ; Yi ZENG
Author Information

1. 100052,中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室
Keywords: Papillomavirus,human; Mammary tumor virus,mouse; Breast neoplasms; Polymerase chain reaction
From: Chinese Journal of Experimental and Clinical Virology 2016;30(2):182-184
CountryChina
Language:Chinese
Abstract: Objective To analyse the presence of human papillomavirus and mouse mammary tumor virus in breast cancer,and expected to provide a possible target for gene therapy of breast cancer.Methods PCR and nested PCR assay were used to detect the HPV L1,HPV16E6,HPV16E7,HPV18E6,HPV18E7 and the enevelop gene of MMTV,while sequence analysis was performed in 76 paraffin embedded tissues of breast cancer.Results The percentage of HPV L1,HPV18 E6,HPV18 E7 and the enevelop gene of MMTV was repectively 9.21％,6.58％,9.21％ and 30.26％,while no HPV16 E6 and HPV16E7 was found in 76 paraffin embedded tissues of breast cancer.Conclusions HPV18 and the enevelop gene of MMTV was existed in breast cancer,there was low connection between HPV and breast cancer,but there may be some correlation between MMTV and breast cancer.This research offers a possible target for gene therapy of breast cancer.