Study on 27 Serum Cytokines in EV71-induced Hand Foot and Mouth Disease in Jinan
10.3760/cma.j.issn.1003-9279.2015.06.005
- VernacularTitle:肠道病毒71感染手足口病患者27种细胞因子的研究
- Author:
Hengyun GUAN
1
;
Xiangjuan HUANG
;
Chunrong WANG
;
Lanzheng LIU
;
Guoliang YANG
;
Xiuyun HAN
;
Hongqi CHENG
Author Information
1. 250021,济南市疾病预防控制中心
- Keywords:
Enterovirus;
Cytokines;
Liquid chip
- From:
Chinese Journal of Experimental and Clinical Virology
2015;29(6):483-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect and analyze the levels of 27 serum cytokines expression in EV71-induced Hand Foot and Mouth Disease(HFMD) in Jinan and revealed their correlations with disease severity based on the platform of liquid chip.Methods 43 serum samples were collected from EV71-infected HFMD patients in Jinan in 2009-2014,including 16 mild cases and 27 severe cases.10 serum specimens from healthy people were also collected as controls.27 serum cytokines were analyzed on Bio-Plex liquid chip platform.Results Compared to healthy controls,22 cytokines expression levels increased in severe groups,including IL-1 ra,IL-2,IL-4,IL-5,IL-6,IL-7,IL-8,IL-9,IL-10,IL-12 (p70),IL-13,IL-15,IL-17,etotaxin,G-CSF,IFN-γ,IP-10,MCP-1,PDGF-BB,MIP-1β,RANTES and TNF-α(P ＜ 0.02).21 cytokines expression levels elevated than healthy controls (P ＜ 0.02).GM-CSF was found decreased levels in both mild and severe groups than in the healthy controls(P ＜ 0.02).The correlation analysis showed that 12 cytokines (IL-1 ra,IL-7,IL-9,IL-10,IL-13,IL-17,etotaxin,IP-10,PDGF-BB,MIP-1β,RANTES and TNF-α) were moderately correlated with the severity of EV71-infected HFMD.GM-CSF was negatively correlated with HFMD.Conclusions Many kinds of serum cytokine changed obviously in the EV71-infected HFMD patients and cytokine levels were closely associated with the severity of HFMD.The analysis of liquid chip for serum cytokines provides an efficiently method.
