Influence of Pixu Fang 1 (Spleen-deficiency Formula 1) on protein and gene expressions of myosin light chain in rats with functional dyspepsia-spleen deficiency pattern
10.3969/j.issn.1006-2157.2017.09.005
- VernacularTitle:脾虚1号方对功能性消化不良脾虚证大鼠胃体组织MLC蛋白与基因表达的影响
- Author:
Lin LYU
1
;
Fengyun WANG
;
Xudong TANG
;
Xiaolan YIN
;
Xiaoshuang SHI
;
Yuanzhi DUAN
Author Information
1. 中国中医科学院西苑医院消化科北京市中医脾胃病研究所 北京100091
- Keywords:
Pixu Fang 1 (Spleen-deficiency Formula 1);
functional dyspepsia;
spleen deficiency pattern;
gastric dynamics;
myosin light chain;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2017;40(9):729-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the intervention of Pixu Fang 1 (Spleen-deficiency Formula 1) to the protein and mRNA expressions of myosin light chain (MLC) in gastric tissue in rats with functional dyspepsia (FD)-spleen deficiency pattern(SDP).Methods SD rats (n =60,aged 7 d) were randomly divided into normal group (n =10) and FD-SDP model group (n =50).The normal group was orally given 2％ sugar solution and FD-SDP model group was given 0.1％ iodoacetamide sugar solution in a dose of O.2 mL each rat a day for 6 d.The FD-SDP model group was fed with normal forage until rats aged 6 weeks and additionally given modified multiple platform method (MMPM) continuously for 14 d.After modeling,FD-SDP model group was divided randomly into model group (distilled water,10 mL/kg · d),domperidone group (3.125 mg/kg · d),low-dose Pixu Fang 1 group (low-dose group,1.275 g/ kg · d),mid-dose Pixu Fang 1 group (mid-dose group,2.550 g/kg · d) and high-dose Pixu Fang 1 group (high-dose group,5.100 g/kg · d,each n =10),and all groups were given orally corresponding medicinal for 14 d.The protein and mRNA expressions of MLC in gastric tissue were detected by using immunohistochemistry technique,Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with normal group,MLC protein expression decreased in model group detected by immunohistochemistry technique and Western blotting,and MLC mRNA expression decreased in model group detected by RT-PCR (P ＜0.01).Compared with model group,MLC protein expression increased in low-dose group,mid-dose group and high-dose group detected by immunohistochemistry technique (P ＜ 0.05,P ＜ 0.01).MLC protein expression increased in mid-dose group and high-dose group detected by Western blotting (P ＜ 0.O1).MLC mRNA expression increased in high-dose group detected RT-PCR (P ＜ 0.01).Conclusion There are decreases of protein and mRNA expressions of MLC in FD-SDP rats,and Pixu Fang 1 can up-regulate these expressions.
