Selective inhibition of HSV - 1 DNA and protein synthesh by antiviral antibiotic 17997
10.3760/cma.j.issn.1003-9279.2000.03.011
- VernacularTitle:抗病毒抗生素17997对HSV1DNA及蛋白质合成的选择性抑制作用
- Author:
Wen CHEN
1
;
YANGMao
;
Peizhen TAO
Author Information
1. Peking Union Medical College, Chinese Academy of MedicalSciences
- Keywords:
Antibiotic;
HSV- 1;
DNA
- From:
Chinese Journal of Experimental and Clinical Virology
2000;14(3):247-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of 17997 on the HSV- 1 DNA and protein synthesis. Meth-ods DNA synthesis was determined by isopycnic separation of cellular and viral DNA in CsCl gradients centrlfu-gation. Protein synthesis was determined by SDS- PAGE and Western Blot. Results The inhibition rates of 0.5μ moL/L, 1.0 μmol/L,2.0 μmol/L and 4.0 μmol/L of 17997 on HSV - 1 DNA synthesis were 63.1% ,74.1%,93.9% and 100% respectively .4.0 μ mol/L of 17997 did not inhibit VERO cell DNA synthesis. The above con-centrations of 17997 did not show any inhibitory effects on cellular protein synthesis.but selectivily inhibited on vi-ral protein synthesis. Conclusion 17997 selectively displayed inhibitive effects on HSV - 1 DNA and protein syn-thesis.
