Accuracy of pooling specimens from different individuals in the detection of genital Chlamydia trachomatis infection: a Meta analysis
10.3760/cma.j.cn112338-20220531-00484
- VernacularTitle:混合不同个体的样本检测生殖道沙眼衣原体感染准确性的Meta分析
- Author:
Tingting JIANG
1
;
Xiangsheng CHEN
Author Information
1. 中国医学科学院/北京协和医学院皮肤病研究所，南京 210042
- Keywords:
Genital;
Chlamydia trachomatis;
Sensitivity;
Specificity;
Meta analysis
- From:
Chinese Journal of Epidemiology
2022;43(12):1995-2001
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the accuracy of pooled specimens from multiple individuals for detection of infection with Chlamydia trachomatis. Methods:By April 2022, PubMed and Embase searched relevant studies published in peer-reviewed journals. The QUADAS-2 scale of a quality assessment tool was used to assess the quality of the studies. A curve of summary receiver operating characteristic was applied as a comprehensive assessment of diagnosed accuracy. A bivariate mixed-effects model was used for overall value merging in sensitivity and specificity. In addition, the subgroup analyses regarding sample type, testing method, and the number of samples per pool were performed.Results:A total of 14 846 subjects were included in the analysis. Three studies were from the United States, three from Canada, three from Denmark, two from Lithuania, two from India, two from the Netherlands, and one from Australia, Russia, and Singapore. Compared with the individual specimens, the pooled specimens of multiple individuals had an overall sensitivity of 0.98 (95% CI: 0.97-0.99) and specificity of 1.00 (95% CI: 1.00-1.00) for the detection of Chlamydia trachomatis. Results from the subgroup showed that the overall sensitivity of ligase chain reaction was significantly higher than that of PCR in the diagnosis of pooled samples. Conclusion:It is concluded from the published studies that the pooled specimens were substantially consistent with the single specimens in detecting infection with Chlamydia trachomatis.
