Effect of zinc ion on the expression of osteoblastic proteins in MC3T3-E1 cells in inflammatory environment
10.3760/cma.j.issn.1002-0098.2016.08.010
- VernacularTitle:锌离子对炎症微环境下成骨细胞成骨相关蛋白表达的影响
- Author:
Jizhou TAN
1
;
Ermin NIE
;
Chunyuan ZHANG
;
Rui JIANG
Author Information
1. 中山大学附属第一医院口腔科
- Keywords:
Zinc sulfate;
Inflammasomes;
Osteoblasts;
Peri-implantitis
- From:
Chinese Journal of Stomatology
2016;51(8):486-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of zinc ion on the expression of osteoblastic proteins.Methods Mice osteoblasts MC3T3-E1 cells were subcultured.Inflammatory environment model was established by tumor necrosis factor α(TNF-αt) at a concentration of 10 mg/L.According to different concentration of Zn2+,the cells were divided into TNF-α group,control group,group A(TNF-α+ 10-4 mol/L Zn2+),group B(TNF-α+ 10-5 mol/L Zn2+),group C(TNF-α+10-6 mol/L Zn2+).After 24,48,and 72 h of culture,cell counting kit-8(CCK-8) assay was used to analyze the proliferation of the cells.ALP activity was examined.Bone morphogenetic protein-2(BMP-2),Runt-related transcription factor 2(RUNX2),Osterix and receptor activator of NF-κB ligand(RANKL) protein levels were determined by Western blotting after 72 h of culture.Results The cells grew by adherence after 24 h.After 72 h,the cells grew dense,and the cells showed long spindle shape or irregular shape.The proliferation of osteoblasts in TNF-α group,group B and group C became lower than that in the control group(P＜0.05),and was not significantly different between group A and the control group(P ＞ 0.05).ALP activity examination demonstrated that the groups cultured for 72 h revealed the highest ALP activity and the most prominent differentation compared with 24 h and 48 h groups.ALP activity was significantly decreased in TNF-α group,group B and group C compared with control group(P＜0.05),but was not significantly different between group A and control group(P＞0.05).The protein levels of BMP-2,RUNX2 and Osterix were significantly decreased in TNF-α group,group B and group C compared with control group(P＜0.05),while showed no significant difference between group A and the control group.Protein level of RANKL was significantly increased in TNF-α group,grope B and group C compared with control group(P＜0.05),while showed no significant difference between group A and control group.Conclusions The concentration of 10-4 mol/L Zn2+ can significantly increase the expression of osteoblastic proteins such as ALP,BMP-2,RUNX2,Osterix and decrease the expression of RANKL in mice osteoblasts in TNF-α inflammatory environment.
