Effects of moxifloxacin in reducing lung injury in mice with Mycoplasma pneumoniae pneumonia
10.13699/j.cnki.1001-6821.2024.19.019
- VernacularTitle:莫西沙星对肺炎支原体肺炎小鼠肺损伤的作用研究
- Author:
Xiao-Hua GUO
1
;
Wei ZHU
;
Qian ZHANG
Author Information
1. 济南市第四人民医院检验科,山东济南 250031
- Keywords:
moxifloxacin;
long non-coding RNA growth arrest specific 5;
apoptosis;
Janus kinase;
signal transducer and activator of transcription;
Mycoplasma pneumoniae pneumonia
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(19):2853-2857
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of moxifloxacin(Moxi)on Mycoplasma pneumoniae pneumonia by down-regulating long non-coding RNA growth inhibition specific gene(lncRNA GAS5)through non-receptor protein tyrosine kinase(JAK)/signaling and transcriptional activator protein(STAT)signaling pathways.Methods BALB/c mice were randomly divided into control group,model group,experimental group and combined group.Mice in model group,experimental group and combined group were treated with nasal drops of Mycoplasma pneumoniae fluid to establish Mycoplasma pneumoniae pneumonia model.By intraperitoneal injection of the experimental group and the combined group of 100 mg·kg-1 moxifloxacin treatment;on the basis,the overexpressed plasmid pc-GAS5 was injected into the tail vein of the combined group.The control group and model group were injected with the same amount of 0.9％NaCl intraperitoneally.The serum levels of interleukin-1 β(IL-1β)and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA);the serum levels of superoxide dismutase(SOD)were detected by biochemical method;and the expression of lncRNA GAS5 in lung tissue was detected by real-time fluorescence quantitative polymerase chain reaction;the protein expression levels of B-cell lymphoma-2(Bcl-2),JAK2 and STAT3 in mouse lung were detected by Western blot.Results The serum IL-1 β content in control group,model group and experimental group were(64.03±14.19),(254.85±63.79)and(157.46±42.93)pg·mL-1,respectively;IL-6 contents were(22.61±5.01),(184.14±39.50)and(132.64±25.47)pg·mL-1;SOD activities were(50.38±11.75),(28.17±6.71)and(37.67±8.09)U·mL-1,respectively;the relative expression levels of lncRNA GAS5 in lung tissues were 1.00±0.16,3.78±0.69 and 2.41±0.54,respectively.The relative expression levels of Bcl-2 protein in control group,model group,experimental group and combined group were 1.00±0.17,0.65±0.14,0.82±0.16 and 0.62±0.13,respectively;phospherylated JAK2/JAK2 ratios were 1.00±0.20,4.98±1.01,2.13±0.52 and 4.21±0.92,respectively;phospherylated STAT3/STAT3 ratios were 1.00±0.18,3.77±0.78,2.65±0.64 and 3.49±0.75,respectively.Compared with the control group,the above indexes in the model group had statistical significance(P＜0.05,P＜0.001).The above indexes in the experimental group were statistically significant compared with those in the model group(P＜0.05,P＜0.01,P＜0.001).There were significant differences between combined group and experimental group(P＜0.05,P＜0.001).Conclusion Moxifloxacin can effectively improve the lung tissue morphology of mice with Mycoplasma pneumoniae pneumonia,regulate the levels of inflammatory factors and oxidative stress damage,and affect apoptosis and JAK/STAT pathway protein expression by down-regulating lncRNA GAS5.
