Protective effects of Ginseng phenolic acids and Ginsenosides on irradiation-induced injury of intestinal crypt cells in mice
10.13699/j.cnki.1001-6821.2024.03.022
- VernacularTitle:人参酚酸和人参皂苷对辐射引起小鼠肠隐窝细胞损伤的保护作用
- Author:
Xiang LI
1
;
Kai SHI
;
Chen LIANG
;
Liang WEI
Author Information
1. 徐州医科大学第一临床医学院,江苏徐州 221004;徐州医科大学江苏省肿瘤生物治疗研究所,江苏徐州 221004
- Keywords:
Ginseng phenolic acid;
Ginsenosides;
ionizing irradiation;
intestinal injury;
intestinal stem cell
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(3):408-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects and mechanism of ginseng phenolic acids(GPAs)and ginsenosides(Gs)on irradiation injury of intestinal epithelial cells.Methods 36 C57BL/6J mice were randomly divided into 12 groups,3 mice in each group:model group,GPAs group and Gs group at 0 h(non-radidtion)and 4 h,24 h and 48 h after radiation.The model group,GPAs group,and Gs group received a single dose of 10 Gy whole-body radiation.At 48.0,24.0,0.5 h before radiation and 0.5 h after radiation,the drug concentration of the GPAs group and the Gs group was 100 mg·kg-1 by gavage,the model group was given the same amount of 0.9％NaCl.The number of proliferating crypt cells was detected by 5-bromo-2'-deoxyuridine(BrdU)immunohistochemistry;Cleaved-caspase 3 immunohistochemical staining was used to detect crypt cell apoptosis;Immunofluorescence staining was used to detect the expression of β-catenin and cluster of differentiation44(CD44)in crypt cells.Results At 4 h after radiation,the BrdU positive cells of model,GPAs and Gs groups were 487.22±113.90,531.56±101.19 and 714.00±84.74;Cleaved-caspase 3 positive cells were 59.89±12.70,41.89±11.70 and 44.11±10.08;β-catenin positive cells were 522.00±69.62,738.56±106.24 and 707.67±117.23;CD44 positive cells were 4.37±1.99,5.54±2.23 and 5.52±2.32.The above indexes of the model group were significantly different from those of the GPAs group and the Gs group(P＜0.05,P＜0.01).Conclusion GPAs and Gs can inhibit the apoptosis of intestinal crypt cells after radiation,significantly improve the proliferation of intestinal crypt cells and protect the survival of intestinal epithelial stem cells after radiation,and repair radiation-induced intestinal injury through Wnt/β-catenin signaling.
