Effect and mechanism of isoliquiritigenin on airway inflammation in damp-heat asthma model rats
10.13699/j.cnki.1001-6821.2023.24.018
- VernacularTitle:异甘草素对湿热哮喘模型大鼠气道炎症的作用及机制
- Author:
Li-Wei LU
1
;
Meng-Lan WANG
;
Xiao-Jun ZHOU
;
Yun-Yun FANG
Author Information
1. 青岛大学附属青岛市海慈医院(青岛市中医医院)儿科,山东青岛 266000
- Keywords:
isoliquiritigenin;
damp-heat asthma;
airway inflammation;
lung function;
inflammatory cell count;
Toll-like receptor 4/nucleus factor-κB pathway
- From:
The Chinese Journal of Clinical Pharmacology
2023;39(24):3628-3632
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of isoliquiritigenin on airway inflammation in damp-heat asthmatic rats by regulating toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)pathway.Methods SD rats were divided into control group,model group,dexamethasone group,experimental-L,-M,-H groups,damp-heat asthma model was established in the other 5 groups except the control group.After successful modeling,the control group and model group were given 0.9％NaCl by gavage,the dexamethasone group was given dexamethasone 0.25 mg,experimental-L,-M,-H groups were given 10,20 and 40 mg·kg-1 of isoglycyrrhizin by gavage,once a day.Asthmatic symptoms were scored after the last sensitization test.The lung function[maximum expiratory flow(PEF),maximum mid-expiratory flow(MMEF),forced vital capacity(FVC)]of rats in each group were detected 24 h after the end of the last administration,and the count of inflammatory cells was detected.Interleukin-13(IL-13)and IL-4 were detected by enzyme-linked immunosorbent assay;the protein expression of TLR4/NF-κB pathway was detected by Western blot.Results The levels of IL-13 in BALF in control group,model group,experimental-H group were(29.71±3.32),(126.37±12.69),(46.94±6.27)ng·L-1;serum IL-13 levels were(17.42±3.11),(85.52±10.71),(38.82±5.89)ng·L-1;IL-4 levels in BALF were(12.38±2.59),(62.17±6.52),(22.50±3.44)ng·L-1;serum IL-4 levels were(10.12±0.42),(44.42±3.69),(16.02±1.62)ng·L-1;TLR4 protein expression were 0.32±0.05,1.23±0.12,0.47±0.06;p-NF-κB p65 protein expression were 0.26±0.03,0.92±0.09,0.39±0.05.The above indexes were statistically significant between the control group and the model group(all P＜0.05);there were statistically significant differences between the model group and experimental-H group(all P＜0.05).Conclusion Isoliquiritigenin can reduce airway inflammation by inhibiting the activation of TLR4/NF-κB pathway in damp-heat asthma model rats.
