Oesophageal cancer cell line TE-1 inhibited the activation,proliferation and apoptosis of co-cultured T cells through PD-1/PD-L1 signaling pathway
10.13699/j.cnki.1001-6821.2023.24.015
- VernacularTitle:食管癌细胞株TE-1经PD-1/PD-L1信号通路抑制共培养T细胞的活化、增殖并促进其凋亡
- Author:
Wen-Jun LU
1
;
Guang-Yuan ZHAO
Author Information
1. 甘肃省武威肿瘤医院胸外科,甘肃武威 733000
- Keywords:
esophageal cancer;
programmed cell death protein-1/programmed cell death protein ligand-1;
co-culture;
T cell
- From:
The Chinese Journal of Clinical Pharmacology
2023;39(24):3613-3617
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of esophageal cancer cell line TE-1 on inhibiting the activation,proliferation,and promoting apoptosis of co-cultured T cells based on the PD-1/PD-L1 signaling pathway.Methods Construct TE-1/PD-L1 cells and TE-1/lock cells through in vitro cell transfection,and detect the expression of PD-L1 after transfection to determine the transfection effect.Four groups of experimental subjects were set up:CD8+T cells+TE-1/PD-L1 cells+IgG antibody group,CD8+T cells+TE-1/mock cells+IgG antibody group,CD8+T cells+TE-1/PD-L1 cells+PD-L1 antibody group,and CD8+T cells+TE-1/PD-L1 cells+PD-1 antibody group.The expression levels of γ-interferon(IFN-γ),tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)secreted by CD8+T cells were compared by enzyme-linked immunosorbent assay(ELISA)among the 4 groups;the proliferation of CD8+T cells among the 4 groups was compared by cell counting kit-8(CCK-8);the apoptosis of CD8+T cells was detected by caspase-3 activity detection kit among the 4 groups.Results The levels of PD-L1 in TE-1 group,TE-1/mock group and TE-1/PD-L1 group were 4.72±0.68,5.04±0.54 and 12.21±1.35,respectively,indicating a significant increase in PD-L1 expression levels in TE-1/PD-L1 cells after transfection.The expression levels of IFN-γ in the supernatant of CD8+T cells+TE-1/PD-L1 cells+IgG antibody group,CD8+T cells+TE-1/mock cells+IgG antibody group,CD8+T cells+TE-1/PD-L1 cells+PD-L1 antibody group and CD8+T cells+TE-1/PD-L1 cells+PD-1 antibody group were(107.80±4.97),(154.00±10.02),(153.00±9.62)and(145.00±17.28)pg·mL-1;the expression levels of TNF-α were(18.80±2.59),(36.60±4.51),(37.00±4.90)and(36.60±3.21)pg·mL-1;the expression levels of IL-10 were(47.20±16.99),(115.00±6.60),(109.60±5.94)and(116.20±8.79)pg·mL-1,respectively.The above indicators of the CD8+T cells+TE-1/PD-L1 cells+IgG antibody group were statistically significant differences from CD8+T cells+TE-1/mock cells+IgG antibody group,CD8+T cells+TE-1/PD-L1 cells+PD-L1 antibody group,and CD8+T cells+TE-1/PD-L1 cells+PD-1 antibody group(all P＜0.05).Conclusion The esophageal cancer cell line TE-1 inhibits the activation,proliferation,and promotes apoptosis of co cultured T cells through the PD-1/PD-L1 signaling pathway.
