Study on the correlation between intestinal flora and gastrointestinal inflammation and gastrointestinal early cancer
10.13699/j.cnki.1001-6821.2023.24.007
- VernacularTitle:肠道菌群与胃肠道炎症和消化道早癌的相关性研究
- Author:
Zhao YU
1
;
Yong DU
;
Yi CHENG
;
Peng WU
;
Xin GAO
Author Information
1. 泰康同济(武汉)医院消化内科,湖北武汉 430050
- Keywords:
gut microbiota;
gastrointestinal inflammation;
early-stage digestive cancer;
dietary habits;
gene mutation
- From:
The Chinese Journal of Clinical Pharmacology
2023;39(24):3575-3579
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of intestinal microbiome in gastrointestinal inflammation and early cancer of digestive tract,and analyze it from multiple factors.Methods Patients with gastrointestinal inflammation and early gastrointestinal cancer were included as treatment group.Healthy volunteers were selected as control group.The diet,lifestyle and other relevant information of the treatment group and control group were recorded,and the intestinal microbial composition of the two groups of samples was compared by high-throughput sequencing technology.The expression levels of sarcoma-causing(Ras),adenomatous polyp coli(APC)and methylcrotonyl-CoA carboxylase(MCC)in rats were detected by enzyme-linked immunosorbent assay.Results The expression levels of Ras in treatment group and control group were(500.00±12.40)and(312.14±9.72)pg·mL-1;the expression levels of APC were(734.25±23.60)and(487.50±16.47)pg·mL-1,respectively;the expression levels of MCC were(434.18±12.50)and(212.36±8.42)pg·mL-1,respectively.The expression levels of Ras,APC and MCC in treatment groups were significantly higher than those in control group,with statistical significance(all P＜0.05).Compared with control group,the abundance of Prevotella,Bacteroidetes and Faecalibacterium in treatment groups increased,and the differences were statistically significant(all P＜0.05).Ace index of treatment group and control group were 223.57±2.04 and 186.52±2.04;Chao index were 235.57±7.04 and 174.52±8.04;Simpson index were 4.93±0.64 and 3.50±0.50,respectively.The indexes of Shannon index were 0.27±0.05 and 0.54±0.13,respectively.The Ace index and Chao index of treatment groups were higher than those of control group,and the differences were statistically significant(all P＜0.05).The results of multiple regression analysis showed that age,body mass index,diet habit,antibiotic use history and disease history were correlated with intestinal flora(all P＜0.05).Conclusion The composition of intestinal microflora is closely related to gastrointestinal inflammation and the risk of early gastrointestinal cancer.
