Determination of sitagliptin in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2018.20.013
- VernacularTitle:液相色谱-串联质谱法测定人血浆中西格列汀的浓度
- Author:
Ai-Lian SHAN
1
;
Rong-Yuan DAI
;
Chao-Qun WANG
;
Hao LI
Author Information
1. 北京大学 第一医院 临床药理研究所
- Keywords:
sitagliptin;
HPLC-MS/MS;
plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(20):2438-2440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC-MS/MS method to deter-minate the concentration of sitagliptin in human plasma .Methods The samples were treated by protein precipitation method .The chromato-graphic column was Venusil ASB C 18(2.1 mm ×50.0 mm,3.0 μm), mobile phase was 100% water contained with 0.1% formic acid -100%acetonitrile contained with 0.1%formic acid, flow rate was 0.40 mL· min-1, column temperature was 50 ℃, injection volume was 20.0 μL, mobile phase.The specificity, standard curve and lower limit of quantitation, precision and recovery , matrix effect and stability of the method were investigated.Results The standard curve of metformin was y=4.73×10 -3x+7.32×10 -4( r=0.998 8 ), and sitagliptin had good linear relationship in 1.00 -800.00 ng· mL-1.The limit of quantifica-tion was 1.00 ng· mL-1.The intra-day RSD and inter-day RSD were less than 12.0%, respectively.The recovery rate of extraction was over 95.9%.Conclusion The method is simple, rapid, accurate and sensi-tive, which is suitable for the determination of sitagliptin in human plasma.
