Protective effects of paeoniflorin against rotenone-induced cell apoptosis and mitochondrial damage in SH-SY5Y cells
10.13699/j.cnki.1001-6821.2018.10.015
- VernacularTitle:芍药苷对鱼藤酮诱导人神经瘤母细胞凋亡和线粒体损伤的保护作用
- Author:
Bao-Ling HU
1
;
Shuang-Shuang LI
;
Ye WAN
;
Chun-Yan GUO
;
Pei-Long YANG
Author Information
1. 河北北方学院药学系
- Keywords:
paeoniflorin;
rotenone;
SH-SY5Y cell;
mitochondrial membrane potential;
α-synuclein;
apoptosis-related protein
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(10):1187-1190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective effects of paeoniflorin against cell death induced by rotenone in human neuroblastoma cell (SH-SY5Y)cells.Methods When the cells reached confluence 70％-80％,the cells were digested and divided into 7 groups:normal group (complete medium),vehicle control group (DMSO),model group (0.25 μmol · L-1 rotenone),positive control group (basis on the model group,plus Madopar 15 μmol · L-1),experimental-L,experimental-M,experimental-H groups (8 × 10-2,0.4,2 μmol · L-1 paeoniflorin).Each group has 6 complex holes.After 24 h administration,the cell survival rate was measured by methyl thiazolyl tetrazolium (MTT).The expression of α-synuclein (α-Syn) and Bcl-2 associated Ⅹ protein(Bax),B cell lymphoma/lewkmia-2 (Bcl-2)were detected by Western blot.Results The cell survial rate in vehicle group,model group,positive control group,experimental-L,experimental-M,experimental-H groups were (97.98 ± 7.92) ％,(70.38 ± 3.00) ％,(77.84 ± 4.10) ％,(78.88 ± 4.53) ％,(78.46 ± 4.87) ％ and (77.58 ± 4.36) ％,respectively.There was significant difference between model and vehicle control group (P ＜ 0.05).The results showed that Madopar and paeoniflorin significantly increased the activity of SH-SY5Y cells versus model group (All P ＜ 0.05).The expression levels of α-Syn in the 6 groups were 0.42 ± 0.24,1.10 ± 0.22,0.23 ± 0.06,(9.0 ± 5.0) × 10-2,0.12 ±0.06,0.21 ± 0.05,respectively.The ratio of Bax/Bcl-2 in the 6 groups were 0.32 ± 0.04,0.49 ± 0.01,(32 ± 0.06) × 10-2,0.22 ± 0.12,(9.0 ± 3.0) × 10-2,(8.0 ± 5.0) × 10-2,respectively.In model group showed an increase in the expression levels of α-Syn and the ratio of Bax/Bcl-2 compared to the vehicle control group (P ＜0.05 and P ＜ 0.01,respectively).Comparison between three concentrations experimental groups and model group,the difference had significantly(all P ＜0.01).The results showed that paeoniflorin can decrease the experession level of α-Syn and decrease the ratio of Bax/Bcl-2,inhibite rotenone induced apoptosis.Conclusion The paeoniflorin had neuroprotective effects on the injury SH-SY5Y cells-induced by rotenone may be related with decreasing the expression of α-Syn and the decreasing ratio of Bax/bcl-2.
