Inhibitory effect of raw malt on the hyperprolactin of female rats induced by risperidone
10.13699/j.cnki.1001-6821.2018.09.024
- VernacularTitle:生麦芽对利培酮所致雌性大鼠高泌乳素血症的抑制作用
- Author:
Cheng-Peng WANG
1
;
Jian-Fei SHI
;
Ming-Fen SONG
;
Yong-Guang WANG
;
Xin LI
Author Information
1. 安徽医科大学安徽省精神医学中心
- Keywords:
raw malt;
risperidone;
prolactin;
hyperprolactinemia
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(9):1092-1094
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of raw malt on contents of prolactin(PRL) in the serum and the of expression of PRL mRNA in hypophysis of female rats hyperprolactin (HPRL) induced by risperidone.Methods The rats were fed with risperidone 0.1 mg · kg-1 · d-1.After 30 d,the HPRL model was prepared.Then the rats were randomly divided into four groups according to their weight,each group had 10 rats:model group and experimental-L,experimental-M,experimental -H groups.And continued to be filled with risperidone 0.1 mg ·kg-1 · d-1 in 4 groups.In addition,the experimental-L,experimental-M,experimental-H groups were also fed with raw malt 10,20,40 g · kg-1 · d-1.Equal volume of 0.9％ NaCl was given to normal group.After 45 d,serum PRL levels in rats were measured by enzyme linked immunosorbent assay.Reverse transcription polymerase chain reaction(RT-PCR) was used to measure hypophysis PRL mRNA expression,and calculate the difference multiples of expression.Results SerumPRLlevels were (16.75 ±2.55),(17.14±1.81),(14.15 ±3.02),(13.65 ±2.28),(14.45 ±1.71) pg· mL-1 in normal group,model group,experimental-L,experimental-M,experimental-H group;comparison between drugs groups and model group,the difference had significantly(all P ＜ 0.05).The hypophysis PRL mRNA expression was 1,then 1.26 ± 1.21,4.29 ± 1.78,5.42 ±0.76,4.50 ±0.29 in normal group,model group,experimental -L,experimental-M,experimental-H group;comparison between three experimental groups and model group,the difference had significantly (all P ＜ 0.05).Conclusion The serum PRL level in female rats can be increased by risperidone.The raw malt can treat the HPRL of female rats caused by risperidone.
