In vivo antibacterial effect of cefathiamidine against mouse septicemia
10.13699/j.cnki.1001-6821.2018.07.036
- VernacularTitle:头孢硫脒对细菌感染小鼠败血症的体内抗菌作用
- Author:
Wei ZHONG
1
;
Yun LI
;
Yuan L(U)
;
Man-Ning LI
;
Jian LIU
;
Chun-Ming QU
Author Information
1. 北京大学第一医院临床药理研究所
- Keywords:
cefathiamidine;
experimental motel of mouse septicemia infected;
in vivo activity;
clinical isolated pathogenic bacteria
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(7):857-860,865
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the in vivo antibacterial effect of cefathiamidinein against mouse septicemia.Methods Experimental model of mouse septicemia was established by intraperitoneally injection with 0.5 mL minimum lethal dose (MLD) bacteria.The 0.2 mL different concentrations of drugs were injected through caudal vein.Cefathiamidine,cefazolin and ampicillin adopted two methods of dose regimen,which are single-dose and two-dose;while,both ceftriaxone and levofloxacin adopted single-dose.The survival time of the infected mouse was monitored for 1-7 d.The 50％,95％ effective doses(ED50,ED95) were determined by the Bi-level integrated system synthesis (BLISS) method.The antibacterial activities between cefiazine and control drugs were compared.In vivo protection experiments were carned out on 3 standard strains and 7 pathogenic strains isolated through single dose.Results The cefathiamidine had good antibacterial activity in vivo against Streptococcus pneumonia and Enterococcus faecalis.The ED50 of single-dose was between 1.43-1.71 mg · kg-1,which was significantly superior to cefazolin and was similar to levofloxacin.According to the results of two -dose regimen,the ED50 values of cefathiamidine against Sreptococcus pneumonia,Staphylococcus aureus and Haemophilus influenza significantly declined,which were between 0.78-14.78 mg · kg-1.However,with regard to Enterococcus faecalis,the ED50 value of two-dose increased compared to that of single-dose,which could be related to the fact that low plasma concentration affected protective effects in vivo.Conclusion Cefathiamidine had a better antibacteria effect in vivo against gram-positive bacteria,especially Streptococcus pneumonia and Enterococcus faecalis.Through the comparison between single-dose and two-dose,it is more reasonable to adopt two-dose or multiple-dose of cefathiamidine with regard to most strains.
