Study of celecoxib in the prevention and treatment of traumatic myositis ossificans in rats
10.13699/j.cnki.1001-6821.2017.23.022
- VernacularTitle:塞来昔布防治大鼠创伤性骨化性肌炎的研究
- Author:
Rong-Dong ZENG
1
;
Ming-Zhong LIU
;
Qiao-Feng CHEN
;
Xiao-Qing CHEN
;
Zhi-Tong XU
;
Zhi-Shan ZHANG
;
Jin-Yang ZHENG
;
Tao LIAN
Author Information
1. 福建医科大学附属泉州市第一医院骨科,福建泉州362000
- Keywords:
celecoxib;
traumatic myositis ossificans;
inflammation factor;
bone morphogenetic protein-4
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(23):2401-2403
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the efficacy and possible molecular mechanisms of celecoxib on the prevention and treatment of traumatic myositis ossificans (TMO) in rats.Methods TMO SD rats were prepared,and 40 rats were randomly divided into control group and test group,20 rats in each group.The test group was given 10 mg · kg-1 · d-1 celecoxib by intragastric administration,qd,and the control group was given an amount of 0.9％ NaCl by intragastric administration,qd.Two groups of rats were given 10 weeks.New bone formation of two groups was detected by X ray examination.Enzyme-linked immunosorbent assay (ELISA) was used to detect the expressions level of tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) and bone morphogenetic protein-4 (BMP-4).Results At the fifth week after operatiorn,the new bone formation in the test group and the control group were 35.00％ (7 cases/ 20 cases) and 70.00％ (14 cases/20 cases),TNF-α levels were (73.6±15.9) and (101.5 ±17.3) ng· L-1,and IL-6 levels were (33.6 ± 5.8) and (52.8 ± 7.2)ng · L-1,the differences were statistically significant (all P ＜ 0.05).At the tenth week after operation,the new bone formation in the test group and the control group were 65.00％ (13 cases/20 cases) and 95.00％ (19 cases/20 cases),TNF-α levels were (55.8-12.7) and (81.7 ± 15.6) ng· L-1,and BMP-4 levels were (4.5 ± 0.3) and (5.3 ± 0.2) ng · L-1,the differences were statistically significant(all P ＜ 0.05).Conclusion Celecoxib has a good preventive and therapeutic effect on TMO,and its mechanism may be related to the inhibition of the level of inflammation and down-regulation of BMP-4 expression.
