Determination the contents of quercetin and kaempfer in Camptotheca acuminata Decne leaves by HPLC
10.13699/j.cnki.1001-6821.2017.16.020
- VernacularTitle:高效液相色谱法测定喜树叶中槲皮素和山柰素的含量
- Author:
Hong-Mei FAN
1
;
Zhi-Hai LIU
;
Yu-Ming LIU
;
Lei L(U)
;
Lin LONG
;
Lan ZOU
Author Information
1. 黔西南州食品药品检验检测中心
- Keywords:
Camptotheca acuminata Decne leaf;
quercetin;
kaempferol;
HPLC
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(16):1585-1587,1609
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC to analyze the content of quercetin and kaempfer in the Camptotheca acuminata Decne leaves.Methods The sample of Camptotheca acuminata Dene leaves were obtained by 5％ hydrochloric acid-methanol The content of quercetin and kaempferol was measured by HPLC with chromatographic column:Hubble C18 (4.6 mm× 250 mm,5 μm),mobile phase:CH3 OH-0.2％ H3PO4(30:70),flow rate:1 mL·min-1,column temperature:30 ℃,detection wavelength:370 nm.The specificity,standard curve and limit of quantification (LOQ),precision and recovery,stability and repeatability were measured.Results The quercetin and kaempferol existed a good linear relation at 5.53-69.08 μg · mL-1 and 3.99-49.90 μg · mL-1,respectively.The LOQ of quercetin and kaempferol was 4.23 and 7.81 ng,respectively.And the average spotting recovery rate of quercetin and kaempferol was 98.94％ and 98.29％,respectively.The RSD values of inter-day and intra-day assays were lower than 0.89％ and 1.04％ for quercetin and kaempferol,respectively.Conclusion This method is an accurate,reliable and convenient method,which is suitable for the quality control of the leaves of Camptotheca acuminata Decne.
