Protective effects of diltiazem on damaged vascular endothelial cells induced by angiotensinⅡ
10.13699/j.cnki.1001-6821.2016.05.019
- VernacularTitle:地尔硫卓对血管紧张素Ⅱ损伤内皮细胞的保护作用研究
- Author:
Qing-Jie CHEN
1
;
Meng YANG
;
Xiao-Feng GUAN
;
Wen-Jun YIN
;
Jiang-Lin WANG
;
Xiao-Cong ZUO
Author Information
1. 中南大学 药学院
- Keywords:
diltiazem;
angiotensin Ⅱ;
humanumbilical vein endothelial cells;
endothelial protection
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(5):443-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of diltiazem ( Dil) on damaged human umbilical vein endothelial cells induced by an-giotensin Ⅱ( Ang -Ⅱ).Methods Human umbilical vein endothelial cells( HUVECs) were cultured in vitro ,with 1 ×10 -6 mol· L-1 Ang-Ⅱas an injury factor simulating the injury model.The cell viability was as-sessed using MTT metabolism assay .The apoptosis rate of HUVECs was detected by flow cytometry , The production of lactate dehydrogenase ( LDH) activity in cell supernatant was detected by colorimetry.The pro-tective effect of diltiazemon damaged HUVECs induced by Ang -Ⅱ was investigated.Results Compared with control group,Ang -Ⅱ(1 ×10-7, 1 ×10-6 , 1 ×10-5 mol · L-1 ) concentration -and time -dependently decrease the cell vitality and the apoptosis rate of HUVECs.Pretreatment with dilconcentration-dependently improved the cell vitality and the apoptosis rate of HUVECs.In addition, compared with the damaged group , diltiazem reduced LDH activity in the cell supernatant.Conclusion Diltiazem has protective effect on the damaged HUVECs induced by Ang -Ⅱ.
