Role of Rho kinase in vascular smooth muscle cell proliferation induced by aldosterone
10.13699/j.cnki.1001-6821.2016.01.013
- VernacularTitle:Rho激酶在醛固酮诱导的血管平滑肌细胞增殖中的作用
- Author:
Chun-Yang MA
1
;
Xiao-Ping WAN
;
Kun YANG
;
Zi-Zhen WANG
;
Chuang-Yi ZHENG
;
Fu-Yue YE
;
Rang WU
Author Information
1. 中南大学 湘雅医院 肝胆肠外科
- Keywords:
aldosterone;
Rho -kinase;
mineralcocorticoid receptor;
vas-cular smooth muscle cell;
cell proliferation
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(1):39-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of Rho kinase in vascular smooth muscle cell ( VSMC) proliferation induced by aldosterone ( ALD) and the effect of mineralcocorticoid receptor (MR).Methods The ex-pressions of Rho -kinase protein were detected by Western blot .VSMC proliferation was measured by [ 3 H] Thymidine incorporation.Results ALD could up-regulate the expressions of Rho -kinase protein in dose-and time-dependent manner , peak at 20 min and 100 nmol · L-1 . Eplerenone , mineralcocorticoid receptor antagonist , could significantly reverse these effects , indicating the activation of Rho -kinase pathway induced by ALD in VSMC was mediated by MR .ALD could significantly induce VSMC proliferation , which could be inhibited by eplerenone and Y-276329 ( Rho-kinase inhibitor ) , indicating the VSMC proliferation induced by ALD was mediated by MR and the activation of Rho -kinase pathway.Conclusion Rho-kinase pathway and MR play an important role in VSMC proliferation induced by ALD .
