Expression of four kinds of miRNAs in isoniazid-induced liver injury rats
10.13699/j.cnki.1001-6821.2015.21.011
- VernacularTitle:4种 miRNAs在异烟肼致大鼠肝损伤肝组织中的表达分析
- Author:
Yu-Hong LI
1
;
Ling-Yan ZHU
;
Chen NIU
;
Yue WANG
;
Shen-Qian TIAN
;
Zhe SHI
;
Yong-Hong XIAO
;
Tie-Sheng HAN
;
Fu-Min FENG
Author Information
1. 华北理工大学 公共卫生学院 河北省煤矿卫生与安全重点实验室
- Keywords:
isoniazide;
liver injury;
miRNAs
- From:
The Chinese Journal of Clinical Pharmacology
2015;(21):2121-2124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of expression level of four miRNAs ( miRNA -122, miRNA -192, miRNA -193, miRNA -125 b1 ) in isoniazid-induced liver injury rats.Methods The rats were randomly divided into six experimental groups and control group.The experimental groups were given isoniazid orally at 55 mg? kg-1? d-1 for 3 , 7 , 10 , 14 , 21 and 28 days and control group was given saline.The pathological changes of liver were observed by light microscope with HE staining.The activity of serum alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) were measured. The expression of miRNAs were determined by real -time fluorescent quantitative PCR. Results With medication time extension, the expression of miRNA-122, miRNA-192 and miRNA-125b1 declined (P<0.01) and significantly lower in 3 days ( P <0.01 ) .While miRNA -193 increased and had a sharp increase at 10 days ( P <0.01 ) . The pathology of liver tissues indicated that liver injury happened at 7 days. The serum activity of ALT, AST showed a trend of increase and had a sharp increase at 10 days ( P <0.01 ) . The abnormal expression of miRNA-122 , miRNA -192 and miRNA -125 b1 were earlier than ALT, AST and pathological changes and had linear correlation with ALT and AST ( P<0.05).In addition, there were linear correlation between four miRNAs ( P <0.05 ) . Conclusion The abnormal expression of miRNA -122 , miRNA-192 and miRNA-125b1 were earlier than ALT and AST, which might be severed as a novel candidate bio-markers for isoniazid-induced liver injury.
