Determinaton the concentration of midazolam and its metabolites in gynaecologic surgical patients and study the distribution of CYP3A4 activity by LC-MS

VernacularTitle:液-质联用法测定中年妇科病人咪达唑仑及其代谢物的血药浓度并研究其细胞色素P450 3A4酶活性分布特征
Author: Zhi-Song LI ¹ ; Wei ZHANG ; Quan-Cheng KAN ; Zhong-Yu WANG ; Hui LU ; Yan-Zi CHANG ; Shu-Sheng ZHANG
Author Information

1. 郑州大学附属第一医院
Keywords: CYP3A4; midazolam; LC/MS
From: The Chinese Journal of Clinical Pharmacology 2010;26(3):213-216
CountryChina
Language:Chinese
Abstract: Objective To study the distribution of CYP3A4 activity in 260 middle-aged gynaecologic surgical patients. Methods Midazolam (MDZ) is used as a probe of eytoehrome P450 3A4 (CYP3A4) activity by a single-point blood measurement. CYP3 A4 activity was measured by plasma ratio of 1'-hydroxymidazolam and midazolam using LC/ MS. Result The mean value of CYP3A4 activity in 260 middle-aged gynaecologic patients (aged 30-50 years) measured was 0. 46 ±0. 14 and 95% confidence interval for mean was 0. 43-0. 48. The range for normal value is 0. 18-0. 73. Conclution CYP3A4 activity is mormal distribution observed in gynaecologic patients. CYP3A4 activity variation has a little effect on the metabolism of commonly used drugs after surgery that are substrates for this enzyme.